BSA4Yeast

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The reference publications are:

Natural variation of chronological aging in the Saccharomyces cerevisiae species reveals diet-dependent mechanisms of life span control.
Jung, P. P.; Zhang, Z.; Paczia, N.; Jaeger, C.; Ignac, T.; May, P. & Linster, C. L.
NPJ Aging and Mechanisms of Disease, 2018, 4, 3
The statistics of bulk segregant analysis using next generation sequencing.
Magwene, P. M.; Willis, J. H. & Kelly, J. K.
PLoS Computational Biology, 2011, 7, e1002255
The genetic architecture of biofilm formation in a clinical isolate of Saccharomyces cerevisiae.
Granek, J. A.; Murray, D.; Kayrkçi, Ö. & Magwene, P. M.
Genetics, Genetics Soc America, 2013, 193, 587-600

The external software tools and versions used in the web-application are:

cutadapt
Cutadapt finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence from your high-throughput sequencing reads.
FastQC (v0.11.4)
A quality control tool for high throughput sequence data.
Samtools (v0.1.18)
Samtools is a suite of programs for interacting with high-throughput sequencing data.
ANNOVAR (2016Feb01)
ANNOVAR is an efficient software tool to utilize update-to-date information to functionally annotate genetic variants.
Flask (0.12.2)
Flask is a microframework for Python based on Werkzeug, Jinja 2 and good intentions.
Celery (4.1.0)
Celery is an asynchronous task queue/job queue based on distributed message passing.
Redis (3.2.10)
Redis is an open source (BSD licensed), in-memory data structure store, used as a database, cache and message broker.
SNAP2
SNAP2 is a method that predicts the effects of single amino acid substitutions in a protein on the protein's function using neural networks.

GitHub repository with the source code used for the pipeline:

BSA4Yeast GitHub repository
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